Accentuate
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Join Trisch as she explores six aspects of negative space design: gradation, reversal, interruption, negative form, ghosting and piecing. Each category features three original quilt designs. Trisch takes you through her thought process and guides you to accentuate the negative in your next quilt.
Two events disrupted the intended course of the experiment and could conceivably have influenced our results. First, a logistical issue delayed our infestation date after we had already adjusted temperatures to their assigned levels, forcing us to correct the temperatures back to the ambient level for fourteen days to minimize the effects of temperature-dependent growth prior to infestation. While it is possible that this initial temperature exposure before the experiment had a minor, exacerbating effect on the subsequent host-parasite interactions in the high-temperature treatments, we think this is unlikely given the two-week re-acclimatization period and the fact that pre-exposure to stressful events tends to prime fish and other animals to subsequent exposure to those stressors, especially in the case of temperature61,62,63,64. If the pre-exposure to high temperatures did, in fact, prime our fish for the experimental temperatures, our results could be seen as conservative. The second event was Hurricane Dorian, which caused temperature fluctuations immediately after infestation in the three temperature treatments that were manually rather than electronically adjusted (Fig. S5). Temperature variability can alter disease transmission and impacts65,66, so these fluctuations, while consistent within temperature treatments (Fig. S5), may have influenced our results. The temperature treatments affected were the three coldest ones, so any negative effect of increased temperature variance on growth, condition, or survival should have diminished the observed temperature-dependent impacts of sea lice rather than accentuated them. 59ce067264